Neutralization and binding activity of a human single-chain antibody to ricin toxin
نویسندگان
چکیده
منابع مشابه
Mechanisms of Neutralization of a Human Anti-Alpha Toxin Antibody
MEDI4893 is a neutralizing human monoclonal antibody that targets alpha toxin (AT), and is currently undergoing evaluation in the field of Staphylococcus aureusmediated diseases. We have solved the crystal structure of MEDI4893 Fab bound to monomeric AT at a resolution of 2.56 Å, and further characterized its epitope using various engineered AT variants. We have found that MEDI4893 recognizes a...
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Ricin toxin binding subunit B (RTB) is one of the subunits of the ricin protein. RTB has been used as adjuvant, but little is known about its mechanism. In this study, we found that RTB increased not only nitric oxide (NO) release, but also tumor necrosis factor (TNF)-α and interleukin (IL)-6 production in mouse macrophage cell line RAW264.7 cells. They subsequently exhibited enhanced ConA-indu...
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Ricin toxin A chain (RTA) was conjugated to monoclonal antibody 791T/36, which was raised originally against human osteogenic sarcoma cell line 791T. The resultant conjugates were characterized and tested for cytotoxicity against a panel of human tumor cell lines representing a defined range of antigenicity with regard to 791T/36. Conjugates were highly cytotoxic for cells expressing high antig...
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ژورنال
عنوان ژورنال: Food and Agricultural Immunology
سال: 2019
ISSN: 0954-0105,1465-3443
DOI: 10.1080/09540105.2019.1698521